Number and average length of sprouts from HUVEC cells treated with the supernatant of miR-205-overexpressing ccRCC cells (Tet-On). a As illustrated by microscopic images (××40 magnification), doxycycline induction (i.e., miR-205 overexpression) compared to EtOH administration was associated with less (b) and shorter vessel sprouts – measured in pixel length by ImageJ (c). Presence and absence of recombinant VEGF within M200 medium served as positive and negative control. **p < 0.01. Method: cells treated with either EtOH or DOX were cultured overnight in hanging drops → incubated for 24 h → spheroids fixed and washed → pictures taken.