Fig. 3.
MF induces miR-205 expression with subsequent downregulation of VEGFA in RCC4 and 786-O cells – mimicking the effects on miR-205 and VEGFA expression in a doxycycline-inducible model of miR-205 overexpression (Tet-On model). a, b qRT-PCR confirmed MF-dependent miR-205 upregulation with subsequent VEGFA in both cell lines. c Western blotting yielded a progressive downregulation of VEGFA protein levels with increasing doses of MF (10 mm and 20 mm) in RCC4 and 786-O cells. d Tet-On model for miR-205 overexpression. e, f qRT-PCR experiments determining miR-205 overexpression and VEGFA downregulation within the miR-205 Tet-On model for RCC4 and 786-O cells. g Diminished VEGFA protein expression in miR-205 overexpressing RCC4 and 786-O cells (Tet-On model). c, g Vinculin was taken as housekeeping protein. **p < 0.01, ***p < 0.001.

MF induces miR-205 expression with subsequent downregulation of VEGFA in RCC4 and 786-O cells – mimicking the effects on miR-205 and VEGFA expression in a doxycycline-inducible model of miR-205 overexpression (Tet-On model). a, b qRT-PCR confirmed MF-dependent miR-205 upregulation with subsequent VEGFA in both cell lines. c Western blotting yielded a progressive downregulation of VEGFA protein levels with increasing doses of MF (10 mm and 20 mm) in RCC4 and 786-O cells. d Tet-On model for miR-205 overexpression. e, f qRT-PCR experiments determining miR-205 overexpression and VEGFA downregulation within the miR-205 Tet-On model for RCC4 and 786-O cells. g Diminished VEGFA protein expression in miR-205 overexpressing RCC4 and 786-O cells (Tet-On model). c, g Vinculin was taken as housekeeping protein. **p < 0.01, ***p < 0.001.

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