... ultracentrifugation. The pelleted prostasome-linked mean aminopeptidase activity was 0.39 U/mg protein (81.9%), with only 0.087 U (18.1%) remaining in the supernatant. The corresponding figures for Γ-glutamyltransferase were 7.89 (60.4%) and 5.17 (39.6%) µkat/g protein, respectively. The different enzyme activities...

.... An aminopeptidase activity was also linked to the prostasomes. This enzyme activity was dramatically inhibited by 2 mmol/l orthophen-antroline. A reactivation of the orthophenantroline-inhibited aminopeptidase activity was possible by adding Zn 2+ to the reaction mixture. Hence, prostasomes contained ATPase as well...