To verify the usefulness of testicular DNA ploidy measurement, we tried some approaches to tumorous and nontumorous testes. Separation of paraffin-embedded specimens into single dissociated nuclei was performed by Hedley’s technique and nuclei were stained with propidium iodide. Control nontumorous testes were clearly separated into the three-peak type with a high G2M percentage and the two-peak type with a low G2M percentage. Among testicular tumors, 25% (8 of 32) had a normal DNA diploid pattern, 15.6% (5 of 32) had an increased DNA tetraploid pattern including DNA polyploidy, and 59.4% (19 of 32) were DNA aneuploid. In detail, 26.3% (5 of 19) of seminomas and 23.1% (3 of 13) of nonseminomas were DNA diploid. Interestingly, seminomas showed a variety of DNA histogram profiles. Among DNA aneuploid tumors, the DNA index of a seminoma was significantly higher than that of a nonseminoma. Among patients who received curative orchiectomy, postoperative metastases did not occur in any of the 8 DNA diploid or 5 tetraploid tumors, but did occur in 4 (21.1%) of 19 DNA aneuploid tumors during an at least 6-year follow-up. Clinicopathological findings were not as predictable as DNA ploidy. Flow cytometry appeared to be useful to characterize testicular tumors.

Keywords:
Testicular neoplasms, Flow cytometry, Ploidy
This content is only available via PDF.
© 1996 S. Karger AG, Basel
1996
Copyright / Drug Dosage / Disclaimer
Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.
You do not currently have access to this content.