Physical separations of different receptor entities by gradient ultracentrifugation or by separations based on ionic strength are interfered with, either by contamination with non-specific binding sites or they are irrelevant because of the low yield of sites after processing. Electrophoretic separation may give a better resolution because of the different nett charges on SHBG and the specific receptors. Isoelectric microfocussing allows the additional differentiation of sites based on the individual isoelectric points of each entity. Using this method, differentiation of at least three different sites in prostate tissue has been achieved.

Keywords:
Receptor purification, Hormone response and resistance, Polyacrylamide gel electrophoresis, Isoelectric microfocussing, Selective inhibition
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© 1979 S. Karger AG, Basel
1979
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