Since the invention of vacuum containers for liquid nitrogen to keep up a constant temperature of ––196.5 °C, and the use of glycerin as cell-protecting substance, it was possible to store human semen at low temperatures for inseminations. Our intention was to keep the damage rate of spermatozoa during the freezing and thawing process as low as possible. Therefore, we tested five different methods. We used different liquids as sperm protectors and tried different procedures of freezing and thawing. The best way for our equipment was the combination of paillettes as sperm containers, a continuous freezing process, thawing at 20 °C, and Matheson’s liquid as sperm-protective substance. Biological and ultrastructural tests of human semen after freezing are improving.

Keywords:
Deep freezing and thawing human semen, Procedures, Protective agents, Percentage of survival
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© 1973 S. Karger AG, Basel
1973
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