Abstract
Bladder cancer (BC), as one of the most common cancers around the world, begins in the inner side of the bladder and is inclined to spread to the remaining parts of the body. Extensive documents have shown that long noncoding RNAs function as stimuli in various cancer types. With regard to LINC00649, there is limited investigation on its role previously. In our research, we discovered that LINC00649 was considerably highly expressed in BC cells and the lack of LINC00649 would cause inactivity in cellular proliferation, migration, and invasion. miR-16-5p turned out to be competitively incorporated by LINC00649 in the upstream or JARID2 downstream. In BC cells, LINC00649 was found to bind with miR-16-5p to increase the expression of JARID2. Overly expressed JARID2 was found to reverse the LINC00649 shortage-mediated suppressive impacts on the cellular process of BC cells. Concisely, it was the first study on the molecular mechanism of LINC00649 in BC. This work detected that LINC00649 enhanced cell proliferation, migration, and invasion of BC cells by acting as a sponge of miR-16-5p and upregulating JARID2, providing novel insight into understating BC.
References
1.
Bladder cancer: diagnosis and management of bladder cancer (c) NICE. Bladder cancer: diagnosis and management of bladder cancer
. BJU Inter
. 2017
;120
(6
):755
–65
.2.
Liu
Y
, Zhao
J
, Zhang
W
, Gan
J
, Hu
C
, Huang
G
, lncRNA GAS5 enhances G1 cell cycle arrest via binding to YBX1 to regulate p21 expression in stomach cancer
. Sci Rep
. 2015
;5
:10159
. .3.
Ding
G
, Li
W
, Liu
J
, Zeng
Y
, Mao
C
, Kang
Y
, LncRNA GHET1 activated by H3K27 acetylation promotes cell tumorigenesis through regulating ATF1 in hepatocellular carcinoma
. Biomed Pharmacother
. 2017
;94
:326
–31
. .4.
Liu
H
, Wang
B
, Zhang
J
, Zhang
S
, Wang
Y
, Zhang
J
, A novel lnc-PCF promotes the proliferation of TGF-β1-activated epithelial cells by targeting miR-344a-5p to regulate map3k11 in pulmonary fibrosis
. Cell Death Dis
. 2017
;8
(10
):e3137
. .5.
Zhong
X
, Lü
M
, Wan
J
, Zhou
T
, Qin
B
. Long noncoding RNA kcna3 inhibits the progression of colorectal carcinoma through down-regulating YAP1 expression
. Biomed Pharmacother
. 2018
;107
:382
–9
. .6.
Ye
G
, Guo
L
, Xing
Y
, Sun
W
, Yuan
M
. Identification of prognostic biomarkers of prostate cancer with long non-coding RNA-mediated competitive endogenous RNA network
. Exp Ther Med
. 2019
;17
(4
):3035
–40
. .7.
He
M
, Lin
Y
, Xu
Y
. Identification of prognostic biomarkers in colorectal cancer using a long non-coding RNA-mediated competitive endogenous RNA network
. Oncol Lett
. 2019
;17
(3
):2687
–94
. .8.
Sang
S
, Zhang
Z
, Qin
S
, Li
C
, Dong
Y
. MicroRNA-16-5p inhibits osteoclastogenesis in giant cell tumor of bone
. BioMed Re Inter
. 2017
;17
:3173547
.9.
Krell
A
, Wolter
M
, Stojcheva
N
, Hertler
C
, Liesenberg
F
, Zapatka
M
, MiR-16-5p is frequently down-regulated in astrocytic gliomas and modulates glioma cell proliferation, apoptosis and response to cytotoxic therapy
. Neuropathol Appl Neurobiol
. 2018
;45
(5
):441
–58
. .10.
Zhang
H
, Yang
K
, Ren
T
, Huang
Y
, Tang
X
, Guo
W
. miR-16-5p inhibits chordoma cell proliferation, invasion and metastasis by targeting Smad3
. Cell Death Dis
. 2018
;9
(6
):680
. .11.
Cheng
B
, Ding
F
, Huang
CY
, Xiao
H
, Fei
FY
, Li
J
. Role of miR-16-5p in the proliferation and metastasis of hepatocellular carcinoma
. Eur Rev Med Pharmacol Sci
. 2019
;23
(1
):137
–45
. .12.
Chavan
S
, Bray
F
, Lortet-Tieulent
J
, Goodman
M
, Jemal
A
. International variations in bladder cancer incidence and mortality
. Eur Urol
. 2014
;66
(1
):59
–73
. .13.
Berdik
C
. Unlocking bladder cancer
. Nature
. 2017
;551
(7679
):S34
–5
. .14.
Wu
XS
, Wang
F
, Li
HF
, Hu
YP
, Jiang
L
, Zhang
F
, LncRNA-PAGBC acts as a microRNA sponge and promotes gallbladder tumorigenesis
. EMBO Rep
. 2017
;18
(10
):1837
–53
. .15.
Kong
Q
, Zhang
S
, Liang
C
, Zhang
Y
, Kong
Q
, Chen
S
, LncRNA XIST functions as a molecular sponge of miR-194-5p to regulate MAPK1 expression in hepatocellular carcinoma cell
. J Cell Biochem
. 2018
;119
(6
):4458
–68
. .16.
Kong
Q
, Qiu
M
. Long noncoding RNA SNHG15 promotes human breast cancer proliferation, migration and invasion by sponging miR-211-3p
. Biochem Biophys Res Commun
. 2018
;495
(2
):1594
–600
. .17.
Liu
Z
, Wang
Y
, Wang
L
, Yao
B
, Sun
L
, Liu
R
, Long non-coding RNA AGAP2-AS1, functioning as a competitive endogenous RNA, upregulates ANXA11 expression by sponging miR-16-5p and promotes proliferation and metastasis in hepatocellular carcinoma
. J Exp Clin Cancer Res
. 2019
;38
(1
):194
. .18.
Xu
Y
, Shen
L
, Li
F
, Yang
J
, Wan
X
, Ouyang
M
. microRNA-16-5p-containing exosomes derived from bone marrow-derived mesenchymal stem cells inhibit proliferation, migration, and invasion, while promoting apoptosis of colorectal cancer cells by downregulating ITGA2
. J Cell Physiol
. 2019
;234
(11
):21380
–94
. .19.
Wang
X
, Lin
Y
, Liu
J
. Long noncoding RNA DLX6AS1 promotes proliferation by acting as a ceRNA targeting miR199a in cervical cancer
. Mol Med Rep
. 2019
;19
(2
):1248
–55
.20.
Lian
Y
, Xiong
F
, Yang
L
, Bo
H
, Gong
Z
, Wang
Y
, Long noncoding RNA AFAP1-AS1 acts as a competing endogenous RNA of miR-423-5p to facilitate nasopharyngeal carcinoma metastasis through regulating the Rho/Rac pathway
. J Exp Clin Cancer Res
. 2018
;37
(1
):253
. .21.
Zhan
Y
, Chen
Z
, Li
Y
, He
A
, He
S
, Gong
Y
, Long non-coding RNA DANCR promotes malignant phenotypes of bladder cancer cells by modulating the miR-149/MSI2 axis as a ceRNA
. J Exp Clin Cancer Res
. 2018
;37
(1
):273
. .22.
Celik
H
, Koh
WK
, Kramer
AC
, Ostrander
EL
, Mallaney
C
, Fisher
DAC
, JARID2 functions as a tumor suppressor in myeloid neoplasms by repressing self-renewal in hematopoietic progenitor cells
. Cancer Cell
. 2018
;34
(5
):741
–e8
. .23.
Kinkel
SA
, Galeev
R
, Flensburg
C
, Keniry
A
, Breslin
K
, Gilan
O
, Jarid2 regulates hematopoietic stem cell function by acting with polycomb repressive complex 2
. Blood
. 2015
;125
(12
):1890
–900
. .24.
Li
Z
, Xu
C
, Gao
M
, Ding
B
, Wei
X
, Ji
N
. Reduced expression of Jumonji AT-rich interactive domain 2 (JARID2) in glioma inhibits tumor growth in vitro and in vivo
. Oncol Res
. 2017
;25
(3
):365
–72
. .25.
Paccez
JD
, Duncan
K
, Sekar
D
, Correa
RG
, Wang
Y
, Gu
X
, Dihydroartemisinin inhibits prostate cancer via JARID2/miR-7/miR-34a-dependent downregulation of Axl
. Oncogenesis
. 2019
;8
(3
):14
. .26.
Wang
X
, Lyu
J
, Ji
A
, Zhang
Q
, Liao
G
. Jarid2 enhances the progression of bladder cancer through regulating PTEN/AKT signaling
. Life Sci
. 2019
;230
:162
–8
. .© 2020 S. Karger AG, Basel
2021
Copyright / Drug Dosage / Disclaimer
Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.
You do not currently have access to this content.
