Background:Escherichia coli O157 is an important food-borne pathogen that can cause diarrhoea, hemorrhagic colitis, and hemolytic uraemic syndrome. Enterobacterial repetitive intergenic consensus sequence PCR (ERIC-PCR) and multilocus sequence typing (MLST) are good methods for molecular typing and the extensive use of antibiotics is a contributing factor to the increasing incidence of antimicrobial-resistant for these strains. Objectives: The aim of this study was to determine the prevalence, antimicrobial resistance, and genetic diversity of E. coli O157 based on the prevalence of urinary tract infection (UTI) in Hubei, China. Results: We obtained 23 (8.07%) E. coli O157 isolates from 285 UTI patients in Hubei, China. All isolates were subjected to antibiotic susceptibility analysis, and molecular typing was performed using ERIC-PCR and MLST. Antimicrobial susceptibility results indicated that most strains were resistant to penicillin (95.65%), chloramphenicol (73.91%), and ampicillin (69.57%). All isolates were discovered to be multiresistant (resistance to more than 3 antibiotics). Genetic variability analysis showed that all of the isolates were grouped into 4 clusters both by ERIC-PCR and MLST. Conclusion: Our findings demonstrated the presence of E. coli O157 in UTIs, provided insights into the dissemination of antibiotic-resistant strains, and improved our knowledge of E. coli O157 risk assessment in UTIs.

Keywords:
Escherichia coli O157, Urinary tract infections, Antibiotic resistance, Enterobacterial repetitive intergenic consensus sequence PCR, Multilocus sequence typing
1.
Ibrahim OMS, Sarhan SR, Hameed AA: In vivo and in vitro antibacterial activities of cranberry extract against E. coli o157:H7 in urinary tract infected rats. Adv Anim Vet Sci 2015;3:233-244.
2.
Wang X, Yan Q, Xia X, et al: Serotypes, virulence factors, and antimicrobial susceptibilities of vaginal and fecal isolates of Escherichia coli from giant pandas. Appl Environ Microbiol 2013;79:5146-5150.
3.
Bien J, Sokolova O, Bozko P: Role of uropathogenic Escherichia coli virulence factors in development of urinary tract infection and kidney damage. Int J Nephrol 2012;2012:681473.
4.
Riley LW, Remis RS, Helgerson SD, et al: Hemorrhagic colitis associated with a rare Escherichia coli serotype. N Engl J Med 1983;308:681-685.
5.
Ogden ID, Hepburn NF, Macrae M, et al: Long-term survival of Escherichia coli O157 on pasture following an outbreak associated with sheep at a scout camp. Lett Appl Microbiol 2002;34:100-104.
6.
Masoumeh N, Abdollah K, Mohammad R, et al: Study prevalence of verotoxigenice E. Coli isolated from urinary tract infections (UTIs) in an iranian children hospital. Open Microbiol J 2012;6:1-4.
7.
Mora A, León SL, Blanco M, et al: Phage types, virulence genes and PFGE profiles of shiga toxin-producing Escherichia coli O157:H7 isolated from raw beef, soft cheese and vegetables in Lima (Peru). Int J Food Microbiol 2007;114:204-210.
8.
Caroppo S, Kroumova V, Grossini E, Ticozzi R, Raimondi A, Fortina G: Phylogenetic relationships, virulence factors and Rep-PCR epidemiological analysis of E. coli from human sources. Microbiol Med 2010;25:78-82.
9.
Casarez EA, Pillai SD, Di Giovanni GD: Genotype diversity of Escherichia coli isolates in natural waters determined by PFGE and ERIC-PCR. Water Res 2007;41:3643-3648.
10.
Babenko DB, Turmukhambetova AA, Aukenov NY: Comparative evaluation of typing methods for E. coli - MLST and serotyping. Sci Health Care 2015;6:77-82.
11.
Zhang S, Zhu X, Wu Q, Zhang J, Xu X, Li H: Prevalence and characterization of Escherichia coli O157 and O157:H7 in retail fresh raw meat in south China. Ann Microbiol 2015;65:1993-1999.
12.
Mulder M, Kiefte-de Jong JC, Goessens WH, et al: Risk factors for resistance to ciprofloxacin in community-acquired urinary tract infections due to Escherichia coli in an elderly population. J Antimicrob Chemother 2016;72:281-289.
13.
Travers K, Barza M: Morbidity of infections aused by antimicrobial-resistant bacteria. Clin Infect Dis 2002;34(suppl 3):S131-S134.
14.
Versalovic J, Koeuth T, Lupski JR: Distribution of repetitive DNA sequences in eubacteria and application to fingerprinting of bacterial genomes. Nucleic Acids Res 1991;19:6823-6831.
15.
Jaureguy F, Landraud L, Passet V, et al: Phylogenetic and genomic diversity of human bacteremic Escherichia coli strains. BMC Genomics 2008;9:560.
16.
Lüdeke CH, Gonzalez-Escalona N, Fischer M, Jones JL: Examination of clinical and environmental Vibrio parahaemolyticus isolates by multi-locus sequence typing (MLST) and multiple-locus variable-number tandem-repeat analysis (MLVA). Front Microbiol 2015;6:564.
17.
Reichwein R, Watson G, Morrison K, et al: Urinary tract infections in hospitalized ischemic stroke patients: source and impact on outcome. Int Stroke Conference 2011;9:E336.
18.
Chaudhry R, Balsara ZR, Madden-Fuentes RJ, et al: Risk factors associated with recurrent urinary tract infection in neurogenic bladders managed by clean intermittent catheterization. Urology 2017;102:213-218.
19.
Laraisla A, Medinapolo J, Alonsoisa M, et al: Urinary infections in patients with catheters in the upper urinary tract: Microbiological Study. Urol Int 2017;98:442-448.
20.
AbongʼO BO, Momba MNB: Prevalence and characterization of Escherichia coli O157:H7 isolates from meat and meat products sold in Amathole District, Eastern Cape Province of South Africa. Food Microbiol 2009;26:173-176.
21.
Djuikoue IC, Njajou O, Kamga HG, et al: Prevalence of CTX-M beta-lactamases in Escherichia coli from community-acquired urinary tract infections and associated risk factors among women in Cameroon. J Epidemiol Res 2017;3:1.
22.
Selim SA, Ahmed SF, Aziz MHA, Zakaria AM, Klena JD, Pangallo D: Prevalence and characterization of shiga-toxin o157:H7 and non-o157:H 7 enterohemorrhagic escherichia coli isolated from different sources. Biotechnol Equip 2014;27:3834-3842.
23.
Hulton CS, Higgins CF, Sharp PM: Eric sequences: a novel family of repetitive elements in the genomes of Escherichia coli, salmonella typhimurium and other enterobacteria. Mol Microbiol 1991;5:825-834.
24.
El-Badawy MF, Tawakol WM, Maghrabi IA, Mansy MS, Shohayeb MM, Ashour MS: Iodometric and molecular detection of ESBL production among clinical isolates of E. Coli fingerprinted by ERIC-PCR: the first egyptian report declares the emergence of E. Coli O25b-ST131clone harboring blages. Microb Drug Resist 2017.
25.
Giammanco GM, Pignato S, Grimont F, et al: Characterization of shiga toxin-producing Escherichia coli o157:H7 isolated in Italy and in France. J Clin Microbiol 2002;40:4619-4624.
26.
Adiri RS, Gophna U, Ron EZ: Multilocus sequence typing (MLST) of Escherichia coli O78 strains. FEMS Microbiol Lett 2003;222:199-203.
27.
Guillouzouic A, Caroff N, Dauvergne S, et al: Mlst typing of Escherichia coli isolates overproducing AmpC {beta}-lactamase. J Antimicrob Chemother 2009;63:1290-1292.
28.
Cabello FC, Godfrey HP, Tomova A, et al: Antimicrobial use in aquaculture re-examined: its relevance to antimicrobial resistance and to animal and human health. Environ Microbiol 2013;15:1917-1942.
29.
Rosello A, Hayward AC, Hopkins S, et al: Impact of long-term care facility residence on the antibiotic resistance of urinary tract Escherichia coli and klebsiella. J Antimicrob Chemother 2017;72:1184-1192.
30.
Ranjbar R, Masoudimanesh M, Dehkordi FS, Jonaidi-jafari N, Rahimi E: Shiga (vero)-toxin producing Escherichia coli isolated from the hospital foods; virulence factors, o-serogroups and antimicrobial resistance properties. Antimicrob Resist Infect Control 2017;6:4.
31.
Munkhdelger Y, Gunregjav N, Dorjpurev A, Juniichiro N, Sarantuya J: Detection of virulence genes, phylogenetic group and antibiotic resistance of uropathogenic Escherichia coli in Mongolia. J Infect Dev Ctries 2017;11:51.
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2017
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