Using a competitive protein-binding method plasma testosterone levels were measured simultaneously in peripheral and spermatic vein blood in 20 sub-fertile men with varicocele (mean value 1.02 ± SD 0.35 µg/100 ml plasma in spermatic and 0.42 ± SD 0.16 µg/100 ml plasma in peripheral vein blood). It was impossible to obtain control values in normal men without varicocele without sacrificing the spermatic vein, values reported by other authors cannot be compared directly because of considerable methodical differences. Nevertheless, impairment of spermatogenesis in varicocele because of a high, local testosterone concentration as a result of the hemodynamic disturbance seems unlikely, as the measured plasma testosterone levels are very low. Determination of these levels in eight adult bulls gave values in good accordance with results reported in the literature.

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