Objective: To investigate, in a rodent model, the influence of an obstructed kidney/ureter on the contralateral organ morphology. Methods: Thirty-six rats were randomly assigned into the following groups: untreated ureteral obstruction group (UO-U, n = 10), submitted to a ligature of the ureter at day 0; UO group (at day 0) followed by nephrectomy after 24 h (UO-N, n = 8); nephrectomy group (N, n = 9), submitted to nephrectomy at day 0; and sham group (S, n = 9), submitted to simulated surgery at day 0. All these procedures were performed on the left kidney/ureter. All animals were euthanized 30 days after surgery, and the right kidneys were collected for stereological analysis. Data were compared using a one-way analysis of variance with Bonferroni’s post test, considering p < 0.05 as significant. Results: The kidney volume, weight, and cortical volume were augmented in groups UO-N and N, compared to group S. Kidneys from the UO-U group showed only a moderate augmentation in kidney weight, with a reduction in the cortical-medullar ratio, compared to the kidneys in group S. No differences in the glomerular volumetric density, volume-weighted glomerular volume, and number of glomeruli were observed among the right kidneys of the studied groups. Conclusion: An obstructed kidney/ureter is more prejudicial to the contralateral kidney morphology than an absent kidney.

Keywords:
Ureteral obstruction, Hydronephrosis, Kidney, Animal models
1.
Quinlan MR, Docherty NG, Watson RW, Fitzpatrick JM: Exploring mechanisms involved in renal tubular sensing of mechanical stretch following ureteric obstruction. Am J Physiol Renal Physiol 2008; 295:F1–F11.
2.
Ucero AC, Benito-Martin A, Izquierdo MC, Sanchez-Nino MD, Sanz AB, Ramos AM, Berzal S, Ruiz-Ortega M, Egido J, Ortiz A: Unilateral ureteral obstruction: beyond obstruction. Int Urol Nephrol 2014; 46: 765–776.
3.
Uchino S, Kellum JA, Bellomo R, Doig GS, Morimatsu H, Morgera S, Schetz M, Tan I, Bouman C, Macedo E, Gibney N, Tolwani A, Ronco C: Acute renal failure in critically ill patients: a multinational, multicenter study. JAMA 2005; 294: 813–818.
4.
Carfagna P, De Cicco Nardone C, De Cicco Nardone A, Testa AC, Scambia G, Marana R, De Cicco Nardone F: The role of transvaginal ultrasound in the evaluation of ureteral involvement in deep endometriosis. Ultrasound Obstet Gynecol 2017, Epub ahead of print.
5.
Harshawardhan Vedpalsingh T, Avinash Vijay J, Vinayak Gorakhnath W, Manoj Vilas B, Sudarshan Omprakash D: Congenital bilateral mid ureteral stenosis: a rare finding. J Clin Diagn Res 2015; 9:PD03–PD04.
6.
Picozzi SC, Marenghi C, Casellato S, Ricci C, Gaeta M, Carmignani L: Management of ureteral calculi and medical expulsive therapy in emergency departments. J Emerg Trauma Shock 2011; 4: 70–76.
7.
Singh I, Strandhoy JW, Assimos DG: Pathophysiology of Urinary Tract Obstruction; in Wein AJ, Kavoussi LR, Novick AC, Partin AW, Peters CA (eds): Campbell-Walsh Urology. Philadelphia, Elsevier Saunders, 2012, pp 1087–1121.
8.
Regazzoni BM, Genton N, Pelet J, Drukker A, Guignard JP: Long-term followup of renal functional reserve capacity after unilateral nephrectomy in childhood. J Urol 1998; 160: 844–848.
9.
Al-Ani A, Al-Jalham K, Ibrahim T, Majzoub A, Al-Rayashi M, Hayati A, Mubarak W, Al-Rayahi J, Khairy AT: Factors determining renal impairment in unilateral ureteral colic secondary to calcular disease: a prospective study. Int Urol Nephrol 2015; 47: 1085–1090.
10.
Damasceno-Ferreira JA, Bechara GR, Costa WS, Pereira-Sampaio MA, Sampaio FJB, Souza DB: The relationship between renal warm ischemia time and glomerular loss. An experimental study in a pig model. Acta Cir Bras 2017; 32: 334–341.
11.
Abreu LADS, Damasceno-Ferreira JA, Costa WS, Pereira-Sampaio MA, Sampaio FJB, de Souza DB: Glomerular loss after renal radiofrequency ablation are comparable to 30 minutes of warm ischemia. J Endourol 2017; 31: 517–521.
12.
Souza DB, Costa WS, Cardoso LE, Benchimol M, Pereira-Sampaio MA, Sampaio FJ: Does prolonged pneumoperitoneum affect the kidney? Oxidative stress, stereological and electron microscopy study in a rat model. Int Braz J Urol 2013; 39: 30–36.
13.
Bechara GR, Damasceno-Ferreira JA, Abreu LAS, Costa WS, Sampaio FJ, Pereira-Sampaio MA, de Souza DB: Glomerular morphology and renal function after warm ischemia by main artery or selective clamping in a porcine model. Urol Int 2017; 993: 262–266.
14.
Benchimol de Souza D, Silva D, Marinho Costa Silva C, Barcellos Sampaio FJ, Silva Costa W, Martins Cortez C: Effects of immobilization stress on kidneys of wistar male rats: a morphometrical and stereological analysis. Kidney Blood Press Res 2011; 34: 424–429.
15.
de Souza DB, de Oliveira LL, da Cruz MC, Abilio EJ, Costa WS, Pereira-Sampaio MA, Sampaio FJ: Laparoscopic partial nephrectomy under warm ischemia reduces the glomerular density in a pig model. J Endourol 2012; 26: 706–710.
16.
Bechara GR, Damasceno-Ferreira JA, Abreu LA, Costa WS, Sampaio FJ, Pereira-Sampaio MA, Souza DB: Glomerular loss after arteriovenous and arterial clamping for renal warm ischemia in a swine model. Acta Cir Bras 2016; 31: 753–758.
17.
Dendooven A, Ishola DA Jr, Nguyen TQ, Van der Giezen DM, Kok RJ, Goldschmeding R, Joles JA: Oxidative stress in obstructive nephropathy. Int J Exp Pathol 2011; 92: 202–210.
18.
Chevalier RL, Forbes MS, Thornhill BA: Ureteral obstruction as a model of renal interstitial fibrosis and obstructive nephropathy. Kidney Int 2009; 75: 1145–1152.
19.
Choi DK, Jung SB, Park BH, Jeong BC, Seo SI, Jeon SS, Lee HM, Choi HY, Jeon HG: Compensatory structural and functional adaptation after radical nephrectomy for renal cell carcinoma according to preoperative stage of chronic kidney disease. J Urol 2015; 194: 910–915.
20.
Choi KH, Lee SR, Hong YK, Park DS: Compensatory structural and functional adaptation after nephrectomy in obese patients according to waist circumference. Urology 2017; 104: 115–121.
21.
Hayslett JP, Kashgarian M, Epstein FH: Functional correlates of compensatory renal hypertrophy. J Clin Invest 1968; 47: 774–799.
22.
Hinman F: Renal counterbalance. Cal West Med 1926; 24: 333–335.
23.
Hayslett JP: Effect of age on compensatory renal growth. Kidney Int 1983; 23: 599–602.
24.
Pabico RC, McKenna BA, Freeman RB: Renal function before and after unilateral nephrectomy in renal donors. Kidney Int 1975; 8: 166–175.
25.
Barry EF, Johns EJ: Intrarenal bradykinin elicits reno-renal reflex sympatho-excitation and renal nerve-dependent fluid retention. Acta Physiol (Oxf) 2015; 213: 731–739.
26.
DiBona GF, Sawin LL: Effect of renal nerve stimulation on NaCl and H2O transport in henle's loop of the rat. Am J Physiol 1982; 243:F576–F580.
27.
Kopp UC, Smith LA: Renorenal reflex responses to renal sensory receptor stimulation in normotension and hypertension. Clin Exp Hypertens A 1987; 9(suppl 1):113–125.
28.
Chevalier RL: Counterbalance in functional adaptation to ureteral obstruction during development. Pediatr Nephrol 1990; 4: 442–444.
29.
el-Dahr SS, Gomez RA, Khare G, Peach MJ, Carey RM, Chevalier RL: Expression of renin and its mrna in the adult rat kidney with chronic ureteral obstruction. Am J Kidney Dis 1990; 15: 575–582.
30.
Berger PM, Diamond JR: Ureteral obstruction as a complication of renal transplantation: a review. J Nephrol 1998; 11: 20–23.
31.
Sandhu C, Patel U: Renal transplantation dysfunction: The role of interventional radiology. Clin Radiol 2002; 57: 772–783.
32.
Greco F, Alba S, Fornara P, Mirone V: Renal transplantation: technical aspects, diagnosis and management of early and late urological complications. Panminerva Med 2016; 58: 294–303.
33.
Kumar S, Ameli-Renani S, Hakim A, Jeon JH, Shrivastava S, Patel U: Ureteral obstruction following renal transplantation: causes, diagnosis and management. Br J Radiol 2014; 87: 20140169.
34.
Hesketh EE, Vernon MA, Ding P, Clay S, Borthwick G, Conway B, Hughes J: A murine model of irreversible and reversible unilateral ureteric obstruction. J Vis Exp 2014; 94.
35.
Tapmeier TT, Brown KL, Tang Z, Sacks SH, Sheerin NS, Wong W: Reimplantation of the ureter after unilateral ureteral obstruction provides a model that allows functional evaluation. Kidney Int 2008; 73: 885–889.
36.
Buys-Goncalves GF, De Souza DB, Sampaio FJ, Pereira-Sampaio MA: Anatomical relationship between the kidney collecting system and the intrarenal arteries in the sheep: contribution for a new urological model. Anat Rec (Hoboken) 2016; 299: 405–411.
37.
de Souza DB, Costa WS, Damasceno-Ferreira JA, Nascimento Junior A, Ascoli FO, Pereira-Sampaio MA, Sampaio FJ: The sheep as a model for healing studies after partial nephrectomy. J Surg Res 2016; 200: 387–391.
38.
Simoes M, de Souza DB, Gallo CB, Pereira-Sampaio MA, Costa WS, Sampaio FJ: Histomorphometric comparison of the human, swine, and ovine collecting systems. Anat Rec (Hoboken) 2016; 299: 967–972.
39.
Miranda FJ, Souza DB, Frazao-Teixeira E, Oliveira FC, Melo JC, Mariano CM, Albernaz AP, Carvalho EC, Souza Wd, DaMatta RA: Experimental infection with the toxoplasma gondii me-49 strain in the brazilian br-1 mini pig is a suitable animal model for human toxoplasmosis. Mem Inst Oswaldo Cruz 2015; 110: 95–100.
40.
Felix-Patricio B, De Souza DB, Gregorio BM, Costa WS, Sampaio FJ: How to quantify penile corpus cavernosum structures with histomorphometry: comparison of two methods. Biomed Res Int 2015; 2015: 832156.
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